Purification and properties of tyrosinase inhibitor from mushroom

Purification of Tyrosinase from Edible Mushroom

Purification and Characterization of Melanogenic Enzyme Tyrosinase from Button Mushroom

Melanogenesis is a biosynthetic pathway for the formation of the pigment melanin in human skin. A key enzyme, tyrosinase, catalyzes the first and only rate-limiting steps in melanogenesis. Since the discovery of its melanogenic properties, tyrosinase has been in prime focus and microbial sources of the enzyme are sought. Agaricus bisporus widely known as the common edible mushroom, it's taking ...

Physicochemical and kinetic properties of mushroom tyrosinase.

Tyrosinase (o-diphenol: O2 oxidoreductase, EC 1.10.3.1) has been purified from a commercial lyophilized powder prepared from edible mushrooms. Specific activities toward DL-dihydroxyphenylalanine and catechol, homogeneity, molecular weight, absorption spectrum, amino acid composition, and copper content of the preparation are comparable to other purified samples of tyrosinase prepared from fres...

The multiple forms of mushroom tyrosinase. Purification and molecular properties of the enzymes.

We have undertaken the purification of mushroom tyrosinase to obtain homogeneous enzyme for a study of its mechanism, which is unknown. Purified mushroom tyrosinases have already been reported (l-9). Some of these preparations were homogeneous, or almost homogeneous, by either electrophoresis or ultracentrifugation; one was homogeneous by both criteria (3, 9). However, there are discrepancies a...

Purification and properties of tyrosinase isoenzymes from hamster melanoma.

Tyrosinase, which catalyzes the formation of melanin from tyrosine (Eq. 1) has only recently been purified to homogeneity from mammalian sources (1, 2). This paper reports a rapid and improved method of purification of one of the several tyrosinase isoenzymes from hamster melanoma and the determination of its amino acid composition. The molecular weights of three of the isoenzymes have been det...